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HomeMy Public PortalAboutFoxPoint Lake Water Quality Monitoring Report 2022 Fox Point Lake 2022 Water Quality Monitoring Report Kaylee MacLeod Blake McNeely February 2023 Prepared for: Municipality of Chester Fox Point Lake Water Quality Monitoring Committee i Fox Point Lake 2022 Water Quality Monitoring Report Contributing Authors: Kaylee MacLeod, MSc, Watersheds & Water Quality Project Coordinator Blake McNeely, BA, Watersheds & Water Quality Team Lead February 2023 Coastal Action 45 School Street, Suite 403 Mahone Bay, N.S., B0J 2E0 Ph: (902) 634-9977 Email: info@coastalaction.org Correct citation for this publication: MacLeod, K. & McNeely, B. (2023). Fox Point Lake 2022 Water Quality Monitoring Report. Mahone Bay, Nova Scotia. This work was supported by: ii Table of Contents List of Figures ........................................................................................................................................................ iii List of Tables ........................................................................................................................................................... v 1.0 Introduction ..................................................................................................................................................... 1 1.1 Fox Point Lake Background ................................................................................................................... 1 1.2 Program Background ............................................................................................................................... 2 1.3 Review of the 2021 Fox Point Lake Water Quality Monitoring Report ................................ 2 2.0 Water Quality Monitoring Results .......................................................................................................... 4 2.1 Physical Water Quality Parameters ................................................................................................... 4 2.1.1 Chlorophyll-α and Phycocyanin ................................................................................................... 4 2.1.2 Stream Discharge............................................................................................................................... 5 2.1.2 Water Temperature and Stratification of Fox Point Lake ................................................. 7 2.1.3 Dissolved Oxygen and Stratification of Fox Point Lake ...................................................... 8 2.1.4 pH ......................................................................................................................................................... 11 2.1.5 Total Dissolved Solids ................................................................................................................... 11 2.2 Chemical Water Quality Parameters ............................................................................................... 12 2.2.1 Total Suspended Solids ................................................................................................................ 12 2.2.2 Total Phosphorus ........................................................................................................................... 13 2.2.3 Total Nitrogen .................................................................................................................................. 15 2.3 Biological Water Quality Parameters ............................................................................................. 17 2.3.1 Fecal Bacteria ................................................................................................................................... 17 2.3.2 Microcystins and Algal Blooms ................................................................................................. 18 3.0 Sediment Sampling .................................................................................................................................... 18 3.1 Metals .......................................................................................................................................................... 18 3.2 Phosphorus ............................................................................................................................................... 21 4.0 Discussion ...................................................................................................................................................... 22 4.1 Algae Blooms in Fox Point Lake ........................................................................................................ 22 4.3 Trophic State of Fox Point Lake ........................................................................................................ 23 4.4 Potential for Nutrient Enrichment of Fox Point Lake .............................................................. 25 5.0 Recommendations ...................................................................................................................................... 27 6.0 References ..................................................................................................................................................... 28 iii List of Figures Figure 1. Drainage basin and sampling sites of Fox Point Lake. ......................................................... 1 Figure 2. Phycocyanin (cells/mL) levels from the 2022 monthly sampling events at the lake sites. The WHO provides two guidelines; Alert level 1 at 20,000 cells/mL, and Alert level 2 at 100,000 cells/mL. ............................................................................................................................................ 5 Figure 3. Single point monthly water discharge from the three streams and the South Culvert monitored at FPL from June-October 2020 along with Environment Canada rainfall data for the same period. ................................................................................................................................... 6 Figure 4. Mean (blue line) stream discharge rates from June to October 2016 to 2022 for comparison. The maximum and minimum ranges of stream discharge are designated by the grey area. .................................................................................................................................................................. 6 Figure 5. Monthly water temperature from the lake and three stream sites at FPL from June-October 2022. The red line indicates the 20oC water temperature threshold for cold- water fish species. ................................................................................................................................................. 7 Figure 6. Monthly water temperature depth profiles from the lake site at FPL from June- October 2022. The red line indicates the 20oC water temperature threshold for cold-water fish species. .............................................................................................................................................................. 8 Figure 7. Monthly DO from the lake and three stream sites at FPL from June-October 2022. The red line indicates the 6.5 mg/L DO minimum for aquatic organisms as set by the CCME. ...................................................................................................................................................................................... 9 Figure 8. Monthly dissolved oxygen depth profiles from the lake site at FPL from June- October 2022. The red line indicates the 6.5 mg/L DO minimum for aquatic organisms, as set by the CCME. ................................................................................................................................................. 10 Figure 9. Monthly pH from the lake and three stream sites at FPL from June-October 2022. The solid red line indicates the 6.5 pH threshold set by CCME, and the dotted red line indicates the 5.0 pH threshold identified by NSSA. .............................................................................. 11 Figure 10. Monthly total dissolved solids from the lake and three stream sites at FPL from June-October 2022. ........................................................................................................................................... 12 Figure 11. Monthly total suspended solids from the lake and three stream sites at FPL from June-October 2022. ........................................................................................................................................... 13 iv Figure 12. Monthly total phosphorus concentrations from the lake, three stream sites and south culvert site at FPL from June-October 2022. Red solid line indicates the 0.03 mg/L MOECC guideline for streams and rivers, while the red dotted line indicates the 0.02 mg/L MOECC guideline for lakes. ............................................................................................................................ 14 Figure 13. Average total phosphorus concentrations from June-October from 2016 to 2022 for comparison. The red solid line indicates the 0.03 mg/L MOECC guideline for streams and rivers, while the red dotted line indicates the 0.02 mg/L MOECC guideline for lakes. .. 15 Figure 14. Monthly total nitrogen concentrations from the lake and three stream sites at FPL from June-October 2022. The red line indicates the 0.9 mg/L guideline for freshwater environments, as set by Dodds and Welch (2000). .............................................................................. 16 Figure 15. Average nitrogen concentrations from June-October 2015 to 2022 for comparison. .......................................................................................................................................................... 16 Figure 16. Monthly fecal bacteria concentrations from the lake and the three stream sites at FPL from June-October 2022. ........................................................................................................................ 17 Figure 17. Phosphorus concentrations in sediment samples from lake and stream sites 2018 – 2022 for comparison. Total phosphorus was not included in the 2021 samples and only the SW Cove site was sampled in 2022. .......................................................................................... 22 Figure 18: Carlson TSI for FPL using the mean Secchi disk depth (transparency), mean chlorophyll-α concentration and mean total phosphorus concentration within FPL in 2022. From Carlson, 1977. .......................................................................................................................................... 24 Figure 19. Comparison of FPL TSI scores from 2015 to 2022 and trophic states, using the Carlson (1977) trophic equations for total phosphorus (TP), chlorophyll-α (Chla), and Secchi disk (Secchi). .......................................................................................................................................... 25 v List of Tables Table 1: Concentration of metals within site sediment samples. Light yellow indicates parameters approaching one of the guidelines, orange indicates an exceedance of ISQG, and red indicates an exceedance of either the PEL or NSEQS guidelines. ............................................ 18 Table 2: Summary of three years of guideline exceedances of metals in sediment samples from SW Cove and South Inlet sampling locations. .............................................................................. 21 Table 3: The results of the 2022 TSI calculations for the 3 associated water quality parameters. Secchi depth was not taken in June, so that parameter is missing from this calculation. ............................................................................................................................................................ 24 Table 4: Nutrient concentrations from surface and at-depth waters (below the thermocline) within FPL. ............................................................................................................................................................ 26 Table 5: Nutrient concentrations of total phosphorus (TP) and total nitrogen (TN) from the two South Inlet sites following rainfall events from 2019 - 2022. ................................................. 27 vi Executive Summary This report outlines the activities and results of the 2022 Fox Point Lake (FPL) water quality monitoring program. This project began as a result of water quality concerns from residents after sedimentation events occurred in the lake following the development of the Aspotogan Ridge Golf Course. In June, August, September and October 2022, volunteers and Coastal Action staff collected water samples at one lake site and three stream sites (an outlet and two inlets). On September 28, 2022 volunteers and staff collected samples for the rainfall event. Sediment samples were also taken at the South West (SW) Cove. An at-depth sample, taken below the thermocline at the lake site, was analyzed for total phosphorus and total nitrogen. Factors measuring or contributing to the production of algae in freshwater, including phycocyanin, total phosphorus and total nitrogen, did not exceed guidelines set by the World Health Organization or Ontario’s Ministry of Environment and Climate Change guidelines, except for the North Inlet, South Inlet and South Culvert which exceeded the threshold of 0.03 mg/L for total phosphorus in rivers and streams at least once in 2022. Surface water temperatures of all sites, except the South Inlet, exceeded or approached the 20oC temperature threshold for cold-water fish species (NSSA 2014) during August. Dissolved oxygen at the lake site stayed above the minimum threshold of 6.5 mg/L set by the Canadian Council of Ministers of the Environment (CCME) for cold -water species (CCME 1999). Some of the stream sites were below this threshold in June, August and October. pH measurements for all sites fell below, or just above the 6.5-pH threshold set by the CCME (2002); however, the acidity of FPL waters is not uncommon for southwest NS lakes, which generally have lower pH values than the 6.5 threshold. It appears that most of the time, the acidity of the waters at FPL poses minimal threat to organisms. E. coli levels at all sites did not exceed or approach recreational guidelines. Despite a spike in levels following the rainfall in October, levels remained below the threshold and returned to 20 CFU/100 mL or less four days later. In the sediment sample from the SW Cove site, arsenic and cadmium exceeded the CCME’s recommended interim sediment quality guidelines (ISQG), while mercury and selenium approached the ISQG and Nova Scotia Environmental Quality Standards (NSEQS) contamination threshold, respectively. Based on the mean depth of transparency (Secchi disk), and mean concentrations of chlorophyll-α and phosphorus, a Trophic State Index (TSI) score has been calculated annually to assess biological productivity. Trophic states range from oligotrophic (low productivity and minimal biomass) to hypereutrophic (high productivity and maximum biomass). The trophic state of FPL in 2015-2019 and 2021 was oligotrophic approaching mesotrophic, while the 2022 TSI results categorize the lake as mesotrophic. vii Within FPL, it appears that external loading of nutrients is affecting the inlet streams more than the lake. Both inlet streams had greater nitrogen and phosphorus concentrations compared to the lake. Recommendations for next steps in Fox Point Lake include using a Sand Wand to remove fine sediment embedded in the Sout Inlet to improve aquatic habitat quality. This would improve the stream habitat for both fish and benthic macro-invertebrates, as well as stream flow. 1 1.0 Introduction The following report summarizes the results from the 2022 monitoring period at Fox Point Lake (FPL). Sample events occurred in June, August, September and October. Samples were not taken in July, therefore the sampling was extended to October according to volunteer availability. A rainfall sample was also taken in October. 1.1 Fox Point Lake Background Fox Point Lake (FPL) is a 1.4 km2 lake located on the Aspotogan Peninsula, within the Municipality of Chester, Nova Scotia (Figure 1). FPL contains 11 small islands and has an average depth of 4.9 m (Beanlands 1980). The lake drains an area of 8 km2, with two inlet streams (the north and south) and one outlet draining into St. Margaret’s Bay. The northern inlet drains a forested region, crossing a wetland before reaching the lake, while the southern inlet runs through the Aspotogan Golf Course. Residential properties, both year-round and seasonal, line the lake perimeter. Figure 1. Drainage basin and sampling sites of Fox Point Lake. 2 1.2 Program Background In 2014, due to concerns from residents about the water quality of FPL, the Municipality of Chester (MOC) created the Fox Point Lake Water Quality Monitoring Committee (WQMC). With development around the lake by Aspotogan Ridge, a 550-acre community with original development plans for 344 residential units and an 18-hole golf course, the rate of rainfall- induced sedimentation plumes in FPL spiked. The sedimentation events, occurring near the southern inlet which drains from the golf course, raised the concerns of citizens for the health of FPL. To monitor the water quality conditions and track changes within the lake, Coastal Action was contracted in 2015 by the Municipality of Chester to join the WQMC and develop and implement a water quality program. The program focuses on four site locations (Figure 1) chosen to monitor water quality incoming, within, and exiting the lake. Monitoring activities within the program are conducted by a small group of trained volunteers, with the support of Coastal Action staff. Further details on the program can be found in the Fox Point Lake Water Quality Monitoring Program (2015), and program results are found in the Fox Point Lake Water Quality Monitoring Reports from 2015-2021; all are available on request from the Municipality of Chester. 1.3 Review of the 2021 Fox Point Lake Water Quality Monitoring Report The 2021 report included data from 2021, as well as a review of water quality changes at the lake from 2015 to 2021. The trophic state of FPL was determined to be oligotrophic and approaching mesotrophic during each sampling year from 2015 through 2019, in 2020 the lake was mesotrophic but returned to oligotrophic in 2021. The increase in chlorophyll-α concentrations suggests the lake may return to the mesotrophic range in future years. Thermal stratification was observed at the lake sample site, while dissolved oxygen (DO) indicated a depletion of oxygen at depth, with concentrations below 3 mg/L recorded in the bottom waters of the lake. 2021 had some of the highest temperatures and lowest DO concentrations of the previous seven years of the study. In all years of the project, the top four meters of the lake exceeded the 20°C threshold, required for cold-water species. In 2021 there were two blooms observed in the lake by volunteers. The first bloom occurred on July 17th, 2021. Results showed no Microcystin-LR present in the sample. The second bloom was detected by volunteers on August 29th, 2021. Results showed a Microcystin-LR concentration of 0.13 µg/L. Blooms were also reported and sampled in 2016, 2017, and 2018 which all confirmed the presence of Microcystin-LR. Blooms were also reported in 2019 and 3 2020; however, Nova Scotia Environment visited the site in 2019 but no bloom was present, and the 2020 sample was lost in transit by UPS. Total phosphorus levels have remained below phosphorus guidelines for most years at the lake, north inlet, and outlet. The South Inlet site exceeded the 0.03 mg/L MOECC stream guideline as it has done from 2015-2021; however, it has been slowly improving despite a slight increase from 2020 to 2021. The south culvert has also exceeded phosphorus guidelines in the three previous years it was sampled. Sediment has been sampled at the Southwest Cove (SWC) and South Inlet (SI) sites since 2017 to test for metal concentrations. At the SWC site, arsenic, cadmium, lead, and mercury exceed or approach ISQG guidelines. At the SWC site, selenium exceeded NSE guidelines each year. For the SI site, mercury exceeded ISQG from 2018 to 2021 and approached the guideline in 2017; while lead levels have decreased and are no longer approaching the guideline. At the SI site, arsenic exceeded NSE guidelines in 2019 and 2021 and exceeded ISQG in the other years. Rainfall was monitored by a volunteer using a rain gauge, then a weather station was installed at the lake in 2021. In 2021, the weather station was used to remotely collect 107 readings, totalling 607 mm of rain. Water levels of the lake (monitored by a volunteer using a staff gauge), remained consistent from 2015 to 2021. Stream discharge rates were highest in 2019 and lowest in 2016, which corresponds with observed rainfall amounts. The trophic state index (TSI) of FPL has consistently been recorded as oligotrophic approaching mesotrophic from 2015-2019; however, the TSI results for 2020 put FPL into the mesotrophic category. The trophic state returned to oligotrophic in 2021. An increasing rate of chlorophyll-α concentrations suggests the lake may return to the mesotrophic range in future years. Water quality data from the North Inlet over the last seven years shows that almost every parameter is rising, except total suspended solids and bacteria which are slightly declining. The stream is becoming more nutrient-rich, with both the temperature and pH increasing. The South Inlet has had varied changes in each parameter. Temperature, dissolved oxygen, total dissolved solids, and total suspended solids are on the rise, while total phosphorus, total nitrogen and bacteria are declining. Water quality parameters for the outlet are all on the rise, except nitrogen which has had a slight downward trend throughout the project. The water quality data from the Lake site show varied changes in every parameter. Dissolved oxygen, pH, total dissolved solids, total nitrogen, and chlorophyll -α are on the rise. Temperature, total suspended solids, total phosphorous, and bacteria are declining. 4 2.0 Water Quality Monitoring Results 2.1 Physical Water Quality Parameters 2.1.1 Chlorophyll-α and Phycocyanin In 2021, a ProDSS Total Algae PC Sensor was purchased by MODL to use on the ProDSS YSI unit owned jointly by MOC and MODL. This probe measures concentrations of chlorophyll-α and phycocyanin present in water. Phycocyanin is a pigment found in cyanobacteria, or blue- green algae, and provides an estimate of total cyanobacteria production. Chlorophyll -α is a pigment produced by all types of algae and provides an estimate of total algae production. Collecting this data over multiple seasons will provide baseline concentrations of phycocyanin in Fox Point Lake, which can vary across waterbodies. Long-term monitoring with this probe, paired with the collection of Microcystin -LR water samples during blooms, will help to identify spikes in phycocyanin concentrations and build a predictive curve for the relationship between the concentrations of these algal pigments and the occurrence of algal blooms in FPL. Algal concentrations are measured as Relative Fluorescence Units (RFU). Phycocyanin RFU units were converted to the total number of cells (Genzoli and Kann 2016). World Health Organization (WHO) provides two guideline levels, ‘alert level 1’ is reached when 20,000 phycocyanin cells/mL are observed, and ‘alert level 2’ is reached when 100,000 phycocyanin cells/mL are observed. At no point were the WHO guidelines exceeded or approached (Figure 2). The data were not collected before, during, or immediately after any known algae blooms. 5 Figure 2. Phycocyanin (cells/mL) levels from the 2022 monthly sampling events at the lake sites . The WHO provides two guidelines; Alert level 1 at 20,000 cells/mL, and Alert level 2 at 100,000 cells/mL. 2.1.2 Stream Discharge Stream discharge rates were monitored at the two inlet sites and the outlet site monthly from June to October 2022. As stream depth and width can affect stream discharge, each stream’s depth, width, velocity, and discharge are measured and calculated on an individual basis. Rainfall data was taken from the Environment Canada historical database for June through October 2022 from the weather station in Halifax (Figure 3). The South Inlet had the lowest and most consistent flow throughout the sampling period, while the North Inlet had a slightly greater discharge in October which corresponds to the rainfall events (Figure 3). The discharge from the Outlet was variable throughout the sampling period. Overall, the rate of discharge had increasing variability at both the South and North Inlet sites from 2018 to 2020, which decreased at both sites from 2021 to 2022 (Figure 4). The outlet has remained relatively stable. -1000 -500 0 500 1000 1500 2000 2500 3000 8-Jun-22 28-Jun-22 18-Jul-22 7-Aug-22 27-Aug-22 16-Sep-22 6-Oct-22 26-Oct-22 15-Nov-22 Ph y c o c y a n i n ( c e l l s / m L ) North Inlet South Inlet Lake Outlet South Culvert 6 Figure 3. Single point monthly water discharge from the three streams and the South Culvert monitored at FPL from June- October 2020 along with Environment Canada rainfall data for the same period. Figure 4. Mean (blue line) stream discharge rates from June to October 2016 to 2022 for comparison. The maximum and minimum ranges of stream discharge are designated by the grey area. 0 10 20 30 40 50 60 70 0.0 0.5 1.0 1.5 2.0 2.5 19-May-22 18-Jun-22 18-Jul-22 17-Aug-22 16-Sep-22 16-Oct-22 15-Nov-22 Ra i n f a l l a m o u n t ( m m ) Di s c h a r g e ( m 3/s ) North Inlet South Inlet Outlet Total Rain (mm) 7 2.1.2 Water Temperature and Stratification of Fox Point Lake Water temperatures from the four FPL sites were recorded monthly during the 2022 program; temperatures ranged from 11.2°C to 23.9°C (Figure 5). The South Inlet was consistently cooler than the other sites throughout the sampling period. The highest temperature recorded was at the Lake site in August. The Lake and Outlet sites exceeded the 20oC temperature threshold for cold-water fish species (Nova Scotia Salmon Association [NSSA] 2014) in June and August. In August, the only site that did not exceed the threshold was the South Inlet. Although the three sites exceeded the threshold, the deeper waters within the lake, the deep cold-water pools within the Outlet, and the one colder inlet stream can provide thermal refuge for fish during the hotter months. However, in previous years, the North Inlet was also a source of cooler water refuge, which might not continue to be the case during the summer months. Figure 5. Monthly water temperature from the lake and three stream sites at FPL from June-October 2022. The red line indicates the 20oC water temperature threshold for cold-water fish species. A monthly water profile was also constructed from within the lake during the 2022 sampling period (Figure 6). Between July to September, the profile shows the development and establishment of a stratified water column, with warmer waters remaining at the surface (epilimnion) and cooler, more-dense waters settling to the lake bottom (hypolimnion). The thermocline, the depth at which the water temperature rapidly changes, is located at 7-m 0 5 10 15 20 25 30 1-Jun-22 1-Jul-22 1-Aug-22 31-Aug-22 1-Oct-22 31-Oct-22 Wa t e r T e m p e r a t u r e (°C) Water Temperature (°C) North Inlet South Inlet Lake Outlet South Culvert 8 water depth in June. Although the surface waters of the lake exceeded the 20oC threshold for cold-water fish in June and August, temperatures were below the threshold in deeper waters. Due to the density differences between the epilimnion and hypolimnion, little mixing occurs within the thermally stratified parts of the lake. September and October had consistent temperatures at all depths, suggesting fall turnover (when the lack of stratification encourages mixing of the waters at all depths of the lake) occurred before the September sampling event. Figure 6. Monthly water temperature depth profiles from the lake site at FPL from June-October 2022. The red line indicates the 20oC water temperature threshold for cold-water fish species. 2.1.3 Dissolved Oxygen and Stratification of Fox Point Lake Dissolved oxygen (DO) within the water was recorded monthly at all four sites, from June to October 2022 (Figure 7). DO ranged from 2.37 mg/L to 9.98 mg/L. The North Inlet consistently had the lowest DO concentrations, while the highest concentrations were recorded within the lake. The low velocity and minimal incline of the North Inlet stream may be factors in the low DO measurements, as these factors limit the water’s ability to engulf oxygen from the air due to the lack of turbulence of the water. The low DO in the North Inlet may negatively affect aquatic organisms. DO is a requirement for the survival of aquatic organisms, with a minimum threshold of 6.5 mg/L set by the 0 5 10 15 20 25 30 0 2 4 6 8 10 12 14 16 18 Te m p e r a t u r e ( °C) Water Depth (m) June August September Rainfall (Oct)October 9 Canadian Council of Ministers of the Environment (CCME) for cold-water species (CCME 1999). Each site, except the lake, had at least one instance of levels below the threshold during the sampling season. The North Inlet was consistently below the threshold except for the September sample and rainfall event in October. The South Inlet and Outlet each had values lower than 6.5 mg/L in August (5.94 mg/L and 5.04 mg/L, respectively). Although fish can survive in low-DO environments for short periods, the low-DO environment in the North Inlet may be causing physiological stress to fish in that stream. The low-DO concentrations in the North Inlet are consistent with previous years. The reduction in DO during the summer is a consistent annual trend due to the decreased ability of warmer waters to dissolve oxygen and the higher DO demand during the growing season, leaving the Lake the only refuge during August. Figure 7. Monthly DO from the lake and three stream sites at FPL from June-October 2022. The red line indicates the 6.5 mg/L DO minimum for aquatic organisms as set by the CCME. DO was also measured within the lake during the monthly water depth profiles (Figure 8). The concentration of DO was uniform throughout the water column in October and the rainfall event. The reduction of DO concentrations within the hypolimnion is associated with minimal mixing of the water column due to the presence of a thermocline in the lake. The lack of mixing and minimal DO inputs from the epilimnion, along with the continued oxygen demand from organisms, results in the decline in DO concentrations in the hypolimnion until 0.0 2.0 4.0 6.0 8.0 10.0 12.0 1-Jun-22 1-Jul-22 1-Aug-22 31-Aug-22 1-Oct-22 31-Oct-22 Di s s o l v e d O x y g e n ( m g / L ) North Inlet South Inlet Lake Outlet South Culvert 10 the water column mixes again during fall turnover (Smith and Bella 1973). By September, fall cooling and mixing had begun bringing DO deeper into the water column with the stratification extending to a 12-m water depth. The DO depth profile within the lake appears to be a clinograde curve, with a negative heterograde curve occurring in August. A clinograde curve occurs when DO decreases in the hypolimnion layer due to high rates of respiration and an increase in decomposition as lake productivity increases predominantly during the summer months. This type of curve was observed primarily in June of this sampling year. A negative heterograde curve can occur when a pocket of low DO occurs mid-profile due to the accumulation and high oxygen demand of decomposing organic matter being caught in the density boundary of the water column (Mackie 2004), which was observed in August (Figure 8). A negative heterograde curve has also been confirmed in previous years in FPL. Figure 8. Monthly dissolved oxygen depth profiles from the lake site at FPL from June-October 2022. The red line indicates the 6.5 mg/L DO minimum for aquatic organisms, as set by the CCME. The summer stratification of the lake may cause stress to organisms within the lake. During August, the hypolimnion’s DO concentrations fell below 6.5 mg/L. The decline in DO concentrations below the CCME threshold occurs at 14 m for June and becomes hypoxic (<2 mg/L) below 15 m. No other months reached hypoxic or anoxic conditions (<1 mg/L). The low dissolved oxygen conditions observed in late summer can reduce the ability to support aquatic life (United States Geological Survey [USGS] 2014; Brylinsky 2004). 0.0 2.0 4.0 6.0 8.0 10.0 12.0 0 2 4 6 8 10 12 14 16 18 Di s s o l v e d O x y g e n ( m g / L ) Water Depth (m) June August September Rainfall (Oct)October 11 2.1.4 pH pH, a measurement of the acidity of a liquid, was measured monthly at each of the four FPL sites (Figure 9). Although most pH measurements fell below the 6.5-pH threshold set by the CCME (CCME 2002), the acidity of the FPL waters is not a significant concern. As Nova Scotia has experienced high amounts of acid precipitation in the past, and its geology limits the replenishment of base cations to soils (NSSA 2015), surface waters within the province are generally lower than the 6.5-pH threshold. In addition, though the FPL sites’ pH values are lower than 6.5 pH, many fish species can survive in waters >5.0-pH (NSSA 2014) and therefore it appears that most of the time (save for the low sub-5.0 pH measurements in the North Inlet and South Inlet, in June and October) the acidity of the waters at FPL pose minimal threat to organisms. Figure 9. Monthly pH from the lake and three stream sites at FPL from June-October 2022. The solid red line indicates the 6.5 pH threshold set by CCME, and the dotted red line indicates the 5.0 pH threshold identified by NSSA. 2.1.5 Total Dissolved Solids Total dissolved solids (TDS) from the four FPL sites sampled monthly from June-October 2022 ranged from 22 mg/L to 53 mg/L (Figure 10). The highest TDS concentrations were consistently measured within the North Inlet, while the remaining three sites never exceeded 35 mg/L. 3 4 5 6 7 8 9 10 1-Jun-22 1-Jul-22 1-Aug-22 31-Aug-22 1-Oct-22 31-Oct-22 pH North Inlet South Inlet Lake Outlet South Culvert 12 Although TDS concentrations within FPL are higher than in other locations and previous years, TDS does not appear to be a problem for aquatic organisms. There is no guideline for TDS set by the CCME for the protection of aquatic health; however, Hinch and Underwood (1985) found that pristine Nova Scotian lakes had an average of 20 mg/L. The lake site within the FPL program had an average of 28.6 mg/L in 2022, suggesting that the lake is not pristine and to some extent affected by sedimentation. The presence of high TDS is not necessarily harmful as dissolved materials can be from both anthropogenic and natural sources. As TDS does not have a guideline for the protection of aquatic organisms, TDS concentrations do not appear to be detrimental to FPL. Figure 10. Monthly total dissolved solids from the lake and three stream sites at FPL from June-October 2022. 2.2 Chemical Water Quality Parameters 2.2.1 Total Suspended Solids Total suspended solids (TSS) were measured as the value of solids suspended in a water column which do not pass through a 45 µm glass fibre filter. For FPL, TSS ranged from <1 mg/L to 12 mg/L, which is the greatest range of TSS values seen throughout this project (Figure 11). The North Inlet had the highest TSS levels of all sites in June and August but was below detection levels in September and the rainfall event. The TSS at the Lake site has remained low for 2022 with a mean concentration of 1.36 mg/L, well below the average 3.0 0 10 20 30 40 50 60 1-Jun-22 1-Jul-22 1-Aug-22 31-Aug-22 1-Oct-22 31-Oct-22 To t a l D i s s o l v e d S o l i d s ( m g / L ) North Inlet South Inlet Lake Outlet South Culvert 13 mg/L background concentration of Nova Scotia lakes reported by Hinch and Underwood (1985). TSS concentrations are not a concern and are consistent with Nova Scotian lakes and previous FPL sampling years. As the CCME has a guideline of a 10 mg/L allowable increase from baseline in waterbodies with TSS ≤ 100 mg/L (CCME 2002), the increases observed in FPL do not appear to be a threat to aquatic organisms. Figure 11. Monthly total suspended solids from the lake and three stream sites at FPL from June-October 2022. 2.2.2 Total Phosphorus Total phosphorus within FPL, monitored and analyzed at BV Labs monthly from June to October 2022, ranged from 0.004 mg/L to 0.085 mg/L (Figure 12). The highest phosphorus concentrations were consistently measured at the South Inlet and were significantly different from the North Inlet, Outlet, and Lake sites. The phosphorus concentration obtained at depth, below the thermocline in the lake, was 0.01 mg/L. Ontario’s Ministry of Environment and Climate Change (MOECC) has established two guidelines for phosphorus in waterbodies: ≤ 0.02 mg/L for lakes, and ≤ 0.03 mg/L for rivers and streams (Ontario’s Ministry of Environment [MOE] 1979). The South Inlet was the only site to consistently exceed the phosphorus guidelines for streams in 2022 as well as the South Culvert site. The North Inlet was 0.03 mg/L during the June sample and exceeded the guideline in August. The phosphorus concentrations of the lake and Outlet sites never exceeded 0.01 mg/L. 0 2 4 6 8 10 12 14 1-Jun-22 1-Jul-22 1-Aug-22 31-Aug-22 1-Oct-22 31-Oct-22 To t a l S u s p e n d e d S o l i d s ( m g / L ) North Inlet South Inlet Lake Outlet South Culvert 14 As mentioned above, the South Inlet site exceeded the 0.03 mg/L MOECC stream guideline for phosphorus during the entire 2022 sampling season. This sample site has exceeded the MOECC stream guideline for phosphorus for each sample obtained throughout this project (2015-2022). As there are few natural phosphorus inputs into the environment, elevated concentrations indicate an anthropogenic source. Although the South Inlet exceeded MOECC stream phosphorus guidelines, phosphorus concentrations within the stream appear to be decreasing over the past seven years (Figure 13). Other sites have maintained relatively consistent total phosphorus levels, except for the North Inlet, which appears to have had a slight increase from 2020 to 2022. Although phosphorus concentrations in the South Inlet are the highest of the four FPL sampling sites, the reduction in phosphorus concentrations suggests that the stream may be slowly recovering from nutrient enrichment. 0.00 0.01 0.02 0.03 0.04 0.05 0.06 0.07 0.08 0.09 8-Jun-22 28-Jun-22 18-Jul-22 7-Aug-22 27-Aug-22 16-Sep-22 6-Oct-22 26-Oct-22 15-Nov-22 To t a l P h o s p h o r o u s ( m g / L ) North Inlet South Inlet Lake Outlet South Culvert At-depth Figure 12. Monthly total phosphorus concentrations from the lake, three stream sites and south culvert site at FPL from June - October 2022. Red solid line indicates the 0.03 mg/L MOECC guideline for streams and rivers, while the red dotted line indicates the 0.02 mg/L MOECC guideline for lakes. 15 Figure 13. Average total phosphorus concentrations from June-October from 2016 to 2022 for comparison. The red solid line indicates the 0.03 mg/L MOECC guideline for streams and rivers, while the red dotted line indicates the 0.02 mg/L MOECC guideline for lakes. 2.2.3 Total Nitrogen Total nitrogen analysis was performed by BV Labs monthly from June to October 2022 for all four FPL sites along with the one South Culvert sample (Figure 14). Total nitrogen ranged from 0.198 mg/L to 0.871 mg/L; the highest nitrogen concentration was recorded at the South Inlet. The nitrogen concentration obtained at depth, below the thermocline in the lake, was 0.389 mg/L. Overall, the nitrogen levels in the lake and outlet have remained consistent since 2016 (Figure 15), while there is an overall slight increase at the South Inlet, North Inlet, and South Culvert sites. 0.000 0.020 0.040 0.060 0.080 0.100 0.120 0.140 0.160 0.180 2015 2016 2017 2018 2019 2020 2021 2022 Av e r a g e P h o s p h o r u s ( m g / L ) Year North Inlet South Inlet Lake Outlet South Culvert 16 Figure 14. Monthly total nitrogen concentrations from the lake and three stream sites at FPL from June-October 2022. The red line indicates the 0.9 mg/L guideline for freshwater environments, as set by Dodds and Welch (2000). Figure 15. Average nitrogen concentrations from June-October 2015 to 2022 for comparison. 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 1-Jun-22 1-Jul-22 1-Aug-22 31-Aug-22 1-Oct-22 31-Oct-22 To t a l N i t r o g e n ( m g / L ) North Inlet South Inlet Lake Outlet South Culvert At-depth 0.000 0.200 0.400 0.600 0.800 1.000 1.200 1.400 2015 2016 2017 2018 2019 2020 2021 2022 Av e r a g e N i t r o g e n ( m g / L ) Year North Inlet South Inlet Lake Outlet South Culvert 17 2.3 Biological Water Quality Parameters 2.3.1 Fecal Bacteria In 2019, the FPL Monitoring Program switched from monitoring fecal coliforms to monitoring Escherichia coli (E. coli) to align with Health Canada’s recommended use of E. coli as the primary indicator of fecal contamination in freshwaters. Health Canada has set primary and secondary recreational contact guidelines for E. coli in freshwaters, ≤400 CFU/100 mL and ≤1000 CFU/100 mL, respectively (Health Canada 2012). Fecal bacteria samples were collected from each FPL site monthly during the 2022 field season (Figure 16). Samples ranged from 2 CFU/100 mL to 160 CFU/100 mL. The North Inlet had the highest E. coli concentration observed in 2022, during the October rainfall sampling event (160 CFU/100 mL). The Lake site had a mean of 10.8 CFU/100 mL throughout the sampling period. During the rainfall-dependent sampling event, bacteria concentrations spiked in the North Inlet, South Inlet, and South Culvert. All other sites also had an increase in bacteria concentrations after the rainfall event; however, levels returned to 20 CFU/100 mL or less at all sites four days after the rainfall. Figure 16. Monthly fecal bacteria concentrations from the lake and the three stream sites at FPL from June-October 2022. 0 20 40 60 80 100 120 140 160 180 1-Jun-22 1-Jul-22 1-Aug-22 31-Aug-22 1-Oct-22 31-Oct-22 E. c o l i (C F U / 1 0 0 m l ) North Inlet South Inlet Lake Outlet South Culvert 18 2.3.2 Microcystins and Algal Blooms The recreational guideline for cyanobacterial toxins – Microcystin-LR is 10 µg/L (Health Canada 2012). This guideline is meant to protect against exposure to microcystins and other toxins that may be present in an algal bloom. Microcystin-LR can persist in aquatic environments after a visible bloom has dissipated (Jones and Orr 1994). Not all algal blooms are toxic cyanobacteria blooms, and Microcystin-LR is only one of the possible toxins in a cyanobacteria bloom. For this reason, every algal bloom should be treated with caution and reported to Nova Scotia Environment (NSE). There were no reports of algae blooms in 2022. There were concerns about substances that looked like a bloom; however, this was not confirmed and no tests were performed. 3.0 Sediment Sampling Sediment sampling from the Southwest side of the lake (known as ‘SW Cove’) occurred on September 28th, 2022. Due to a sampling error, no sample was taken from the South Inlet in 2022. The substrate was analyzed for metals, phosphorus, and orthophosphate, to assess the risk of internal nutrient loading within the lake and the potential risk from the accumulation of metals within the sediments (Tables 1 & 2). 3.1 Metals Table 1: Concentration of metals within site sediment samples. Light yellow indicates parameters approaching one of the guidelines, orange indicates an exceedance of ISQG, and red indicates an exceedance of either the PEL or NSEQS guidelines. Sediment Concentration Guidelines Metals UNITS SW Cove ISQG PEL NS 2022 Acid Extractable Aluminum (Al) mg/kg 6400 Acid Extractable Antimony (Sb) mg/kg ND 25 Acid Extractable Arsenic (As) mg/kg 8 5.9 17 17 Acid Extractable Barium (Ba) mg/kg 35 19 Acid Extractable Beryllium (Be) mg/kg 1.6 Acid Extractable Bismuth (Bi) mg/kg ND Acid Extractable Boron (B) mg/kg ND Acid Extractable Cadmium (Cd) mg/kg 0.63 0.6 3.5 3.5 Acid Extractable Chromium (Cr) mg/kg 3.2 37.3 90 90 Acid Extractable Cobalt (Co) mg/kg 1.1 Acid Extractable Copper (Cu) mg/kg 5.7 35.7 197 197 Acid Extractable Iron (Fe) mg/kg 2900 47,766 Acid Extractable Lead (Pb) mg/kg 9 35 91.3 91.3 Acid Extractable Lithium (Li) mg/kg 5.8 Acid Extractable Manganese (Mn) mg/kg 160 1,100 Acid Extractable Mercury (Hg) mg/kg 0.14 0.17 0.486 0.486 Acid Extractable Molybdenum (Mo) mg/kg ND Acid Extractable Nickel (Ni) mg/kg 2.2 75 Acid Extractable Phosphorus (P) mg/kg 510 Acid Extractable Rubidium (Rb) mg/kg 5.2 Acid Extractable Selenium (Se) mg/kg 1.7 2 Acid Extractable Silver (Ag) mg/kg ND 1 Acid Extractable Strontium (Sr) mg/kg 13 Acid Extractable Thallium (Tl) mg/kg ND Acid Extractable Tin (Sn) mg/kg 1.1 20 Acid Extractable Uranium (U) mg/kg 10 Acid Extractable Vanadium (V) mg/kg 6.9 Acid Extractable Zinc (Zn) mg/kg 31 123 315 315 Orthophosphate (P) mg/kg 0.47 ND = Not Detected Three guidelines are used for comparison for the sediment analysis; the CCME’s recommended Interim Sediment Quality Guideline (ISQG), the CCME’s Probable Effect Level (PEL), and the Nova Scotia Environmental Quality Standards (NSEQS) contamination threshold. The results for 2022 at SW Cove indicate that arsenic and cadmium exceeded the ISQG guidelines. Mercury and selenium were approaching ISQG and NSEQS guidelines, respectively (CCME 2001; NSE 2014). Arsenic, cadmium, lead, and selenium were lower than 2021 levels (Table 2). Mercury levels increased by 0.1 mg/kg in 2022. Selenium, which exceeded the NS guidelines last year, fell below the threshold in 2022. 21 Table 2: Summary of three years of guideline exceedances of metals in sediment samples from SW Cove and South Inlet sampling locations. SW Cove South Inlet Guidelines UNIT S 2018 2019 2020 2021 2022 2017 2018 2019 2020 2021 ISQG PEL NS Acid Extractable Arsenic (As) mg/k g 6 6.1 9.1 20 8 7.9 10 22 9.8 35 5.9 17 17 Acid Extractable Cadmium (Cd) mg/k g ND 0.8 0.48 1.6 0.63 ND 0.4 0.37 ND ND 0.6 3.5 3.5 Acid Extractable Lead (Pb) mg/k g 2.6 50 61 21 9 17 33 31 21 21 35 91.3 91.3 Acid Extractable Mercury (Hg) mg/k g ND 0.16 0.23 0.13 0.14 0.12 0.21 0.17 0.17 0.19 0.17 0.48 6 0.48 6 Acid Extractable Selenium (Se) mg/k g ND 2.7 2.1 3.3 1.7 ND 1.1 1 0.71 0.9 2 3.2 Phosphorus Concentrations of both acid-extractable (total) phosphorus and bioavailable orthophosphate were analyzed within the sediment substrate from SW Cove. Within SW Cove, the concentration of orthophosphate was 0.16 mg/kg in 2021 and increased to 0.47 mg/kg in 2022 (0.09% of total phosphorus concentrations) (Figure 17). 22 Figure 17. Phosphorus concentrations in sediment samples from lake and stream sites 2018 – 2022 for comparison. Total phosphorus was not included in the 2021 samples and only the SW Cove site was sampled in 2022. There was an increase in bioavailable phosphorus, despite a decrease in total phosphorus from 2020 to 2022. This suggests that there is more bioavailable phosphorus within the lake, which can result in nutrient enrichment during fall turnover if the available orthophosphate stores increase and are not assimilated. The total phosphorus concentrations from both the SW Cove and South Inlet sites suggest marginally polluted sediment from 2019 to 2020. According to Ontario’s provincial sediment quality guidelines, pollution can range from clean/marginally polluted (‘lowest effect level’) at 600 mg/kg of phosphorus to heavily contaminated (‘severe effect level’) at >2000 mg/kg of phosphorus in sediment (Ontario MOE 2008). The decrease in total phosphorus at the SW Cove site in 2022, results in levels below the 600 mg/kg boundary. 4.0 Discussion 4.1 Algae Blooms in Fox Point Lake There were no reported algae blooms in 2022 at Fox Point Lake. NS Environment’s current system of notifying lake residents of potentially harmful algae blooms is reactive and can be ineffective. NSE responds to reports of suspected blooms but inspectors are not always able to respond in time to witness the bloom. NSE rarely collects water samples for analysis and often has to post precautionary advisories based on the appearance of a bloom in photographs from residents. Lake closure advisories are posted via Twitter and other online locations. Many residents of FPL do not have internet access at the lake. An NSE advisory posted via Twitter in the summer of 2021 did not reach the majority of lake residents. 23 Microcystin is not the only toxin produced by cyanobacteria. Anatoxins, Cylindrospermopsins, Nodularins, Saxitoxins, Dermatoxtoxins, and other irritant toxins are also produced by cyanobacteria (Health Canada 2012). The majority of commercial labs in Canada do not test for these toxins. This means that the absence of Microcystin-LR in a water sample does not mean that a bloom does not contain other toxins. Because of this, lake residents should be made aware of all blooms and treat all blooms with the same level of caution. As algal blooms can be induced and intensified by increases in nutrients to ecosystems (whether naturally from the mixing of waters or anthropogenically from pollution), trends in algal blooms are hard to predict and can vary spatially. Increases in total nitrogen and phosphorus concentrations in FPL increase the potential for blooms to occur. The literature predicts increases in both size and frequency of blooms, globally, in the future (Michalak et al. 2013). Algal blooms should continue to be monitored and tested within FPL, with residents made aware of algal bloom causes, health effects, precautions to take, and the reporting procedure if a bloom occurs. 4.3 Trophic State of Fox Point Lake Using various water parameters, the biological productivity of a lake can be assessed and monitored for changes over time. Based on the mean depth of transparency (Secchi disk), and mean concentrations of chlorophyll-α and phosphorus, a Trophic State Index (TSI) score can be calculated using the Carlson (1977) equations (Equations 1, 2, and 3) and averaging the results (Figure 18). By calculating a TSI of a waterbody, the biological state (trophic state) of the water and how it changes over time can be monitored. Trophic states range from oligotrophic (low productivity and minimal biomass) to hypereutrophic (high productivity and maximum biomass). Equation 1: 𝑆𝑆𝐼 (𝑆𝑐𝑐𝑐𝑖ℎ 𝑐�ℎ𝑟𝑘)=60 −14.41 × ln(𝑀𝑐𝑎𝑘 𝑆𝑐𝑐𝑐𝑖ℎ 𝑐�ℎ𝑟𝑘 [𝑘]) Equation 2: 𝑆𝑆𝐼 (𝑐�𝑘𝑘𝑟𝑘𝑘�𝑦𝑘𝑘 𝐴)=30.6 +9.81 × ln(𝑀𝑐𝑎𝑘 𝑐�𝑘𝑘𝑟𝑘𝑘�𝑦𝑘𝑘 𝑎 [𝜇𝑔 𝐿]) Equation 3: 𝑆𝑆𝐼 (𝑟𝑘𝑟𝑎𝑘 𝑘�𝑘𝑟𝑘�𝑘𝑟𝑟𝑟)=4.15 +14.42 × ln(𝑀𝑐𝑎𝑘 𝑟𝑘𝑟𝑎𝑘 𝑘�𝑘𝑟𝑘�𝑘𝑟𝑟𝑟 [𝜇𝑔 𝐿]) 24 Table 3: The results of the 2022 TSI calculations for the 3 associated water quality parameters. Secchi depth was not taken in June, so that parameter is missing from this calculation. Parameter Calculated TSI Value Secchi (Transparency) 50.17 Chl α 44.59 Phosphorous 29.99 TSI Result 41.58 Figure 18: Carlson TSI for FPL using the mean Secchi disk depth (transparency), mean chlorophyll-α concentration and mean total phosphorus concentration within FPL in 2022. From Carlson, 1977. For FPL, the trophic state was determined to be oligotrophic approaching mesotrophic from 2015-2019 and 2021, however, the TSI results for 2022 put FPL into the mesotrophic category (Figure 19). Although the transparency via Secchi disk is not an exact indication of a waterbody’s productivity due to interference by factors other than biomass (such as 25 suspended particles within the water column) (NSSA 2014; United States Environmental Protection Agency [US EPA] 2002), the continuing increase in chlorophyll-a concentration over several years, and despite the slight decrease in total phosphorus concentrations in the water, confirms this shift to a mesotrophic state. The eutrophication process is driven initially by catchment processes that contribute excess nutrients, particularly phosphorus, to a waterbody and increases the probability of the occurrence of cyanobacterial blooms (Necombe et al. 2010). Figure 19. Comparison of FPL TSI scores from 2015 to 2022 and trophic states, using the Carlson (1977) trophic equations for total phosphorus (TP), chlorophyll-α (Chla), and Secchi disk (Secchi). 4.4 Potential for Nutrient Enrichment of Fox Point Lake Excessive nutrients entering FPL can be detrimental to the health of the lake and will change the lake’s trophic status. Nutrient enrichment can be from both external and internal loading sources. External sources can be natural (wildlife waste, plant decomposition, etc.) or anthropogenic (septic tank malfunction, fertilizer application, livestock waste, composter leachate, etc.) (Sereda et al. 2008; Wetzel 1990; Dion et al. 1983). Internal sources come from within the lake, whether nutrients trapped within the lake substrate (i.e., orthophosphate) or nutrients trapped in the water below the thermocline, and therefore are unable to be mixed and dispersed throughout the lake (Sondergaard et al. 2003; Kennedy and Walker 1990). 26 Within FPL, external loading appears to affect the inlet streams more than the lake. Both inlet streams had greater nitrogen and phosphorus concentrations compared to the lake. The elevated nutrient concentrations of these two inlet streams suggest a level of pollution related to nutrients, particularly phosphorus entering the South Inlet from nearby sources. Nutrient loading within the two inlets is further increased during rainstorms via overland flow. Further increases in nutrients from either stream may affect the delicate balance within the lake and cause eutrophication. Internal loading poses a risk to nutrient enrichment and eutrophication in FPL. Within the water column, there are comparable nitrogen concentrations and an increase in phosphorus concentrations below the thermocline compared to the surface waters (Table 4). When fall turnover occurs, the redistribution of these elevated levels of nutrients results in a source of internal loading and may cause eutrophication of the lake. Nutrients in sediments are an additional internal loading source. In addition, the release of phosphorus from sediment is not limited to lakes and can also occur in streams; the South Inlet’s marginally phosphorus- polluted stream acts as an additional source of nutrients, which may impact the lake. Table 4: Nutrient concentrations from surface and at-depth waters (below the thermocline) within FPL. Surface Waters At-Depth Waters 2019 2020 2021 2022 2019 2020 2021 2022 Phosphorus Concentrations (mg/L) 0.007 0.261 0.007 0.006 0.012 0.246 0.010 0.010 Nitrogen Concentrations (mg/L) 0.007 0.304 0.267 0.257 0.014 0.288 0.572 0.389 The residential development occurring along the South Inlet may to be impacting nutrient enrichment within FPL (Table 5) and warrants further investigation. The barn located on the property is used to house animals, with their waste stored on-site. As animal waste contains bacteria and nutrients (Vanni 2002), these can be flushed into the South Inlet and eventually the lake if not properly contained. The water samples from the South Inlet and Secondary Southern site, below this property, showed little difference in phosphorous and nitrogen between the two sites (Table 5). This suggests that there is little to no impact from the farm in 2022. 27 Table 5: Nutrient concentrations of total phosphorus (TP) and total nitrogen (TN) from the two South Inlet sites following rainfall events from 2019 - 2022. South Inlet South Culvert Site Difference 2019 2020 2021 2022 2019 2020 2021 2022 2019 2020 2021 2022 TP (mg/L) 0.049 0.645 0.063 0.058 0.049 0.056 0.063 0.067 0.000 -0.589 0.000 -0.009 TN (mg/L) 0.065 0.714 0.461 0.835 0.661 0.691 0.461 0.82 0.596 -0.023 0.000 0.015 5.0 Recommendations Below are recommendations for the next steps in Fox Point Lake. 1) Review and discuss the continuation of FPL water quality monitoring for 2023. Additional project activities that we suggest to be considered are: • Use of a Sand Wand to remove fine sediment embedded in the South Inlet to improve aquatic habitat quality. After multiple years of sedimentation runoff, the stream bed has become heavily sedimented and holds excess nutrients that may continue to impact the lake for many years. • Assess South Inlet for other aquatic habitat qualities that may need to be remediated (i.e., debris jams, unstable banks, or point-source pollutants such as agriculture practices within the riparian zone). Excess sand and silt in streams can reduce habitat for aquatic invertebrates, as well as widen the stream and reduce water flow (Adopt a Stream 2020). Removing the fine materials from the South Inlet stream bed could improve fish spawning and invertebrate habitats, as well as improve water flow and stream health over time. 28 6.0 References Beanlands, D.I. 1980. Surveys of Ten Lakes in Guysborough, Halifax, Hants, and Lunenburg Counties, Nova Scotia, 1978. Freshwater and Anadromous Division Resource Branch. Canadian Data Report of Fisheries and Aquatic Sciences No. 192. Brylinsky, M. 2004. User’s Manual for Prediction of Phosphorus Concentration in Nova Scotia Lakes: A Tool for Decision Making. Version 1.0. Acadia Centre for Estuarine Research, Acadia University. 82 p. Canadian Council of Ministers of the Environment (CCME). 1999. Canadian water quality guidelines for the protection of aquatic life: Dissolved oxygen (Freshwater). In: Canadian environmental quality guidelines, 1999, Canadian Council of Ministers of the Environment, Winnipeg. Canadian Council of Ministers of the Environment (CCME). 2001. Canadian sediment quality guidelines for the protection of aquatic life: Introduction. Updated. In: Canadian environmental quality guidelines, 1999, Canadian Council of Ministers of the Environment, Winnipeg. Canadian Council of Ministers of the Environment (CCME). 2002. Canadian water quality guidelines for the protection of aquatic life: Total particulate matter. In: Canadian environmental quality guidelines, 1999, Canadian Council of Ministers of the Environment, Winnipeg. Canadian Council of Ministers of the Environment (CCME). 2004. Canadian water quality guidelines for the protection of aquatic life: phosphorus: Canadian guidance framework for the management of freshwater systems. In: Canadian environmental quality guidelines, 2004, Canadian council of ministers of the environment, Winnipeg. Carlson, R. E. 1977. A trophic state index for lakes. Limnology and oceanography, 22(2), 361-369. Dion, N. P., Sumioka, S. S., and Winter, T. C. 1983. General hydrology and external sources of nutrients affecting Pine Lake, King County, Washington. US Department of the Interior, US Geological Survey. Dodds, W.K. and Welch, E.B. 2000. Establishing nutrient criteria in streams. J.N.Am.Benthol.Soc.19(1), 186-196. Health Canada. 2012. Guidelines for Canadian Recreational Water Quality, Third Edition. Water Air, and Climate Change Bureau, Healthy Environments and Consumer Safety Branch, Health Canada, Ottawa, Ontario. (Catalogue No H129-15/2012E). Hinch, P.R. and Underwood, J.K. 1985. A study of aquatic conditions in Lake Echo during 1984. N.S. Dept. Env. Lib. L192.1 85/00 C2. 38 p. 29 Jones, G.J. and Orr, P.T. 1994. Release and degradation of microcystin following algicide treatment of a Microcystis aeruginosa bloom in a recreational lake, as determined by HPLC and protein phosphatase inhibition assay. Water Res., 28: 871–876. Kennedy, R. H., and Walker, W. W. 1990. Reservoir nutrient dynamics. Reservoir limnology: ecological perspectives, 109-131. Mackie, G. 2004. Applied Aquatic Ecosystem Concepts. 2d ed. Dubuque, Iowa. Kendall/Hunt Publishing Company. Michalak, A.M., Anderson, E.J., Beletsky, D., Boland, S., et al. 2013. Record-setting algal bloom in Lake Erie caused by agricultural and meteorological trends consistent with expected future conditions. Proceedings of the National Academy of Sciences, 201216006. Necombe, G., House, J., House, Ho, L., Baker P. and Burch M. 2010. Management Strategies for Cyanobacteria (Blue-Green Algae): A Guide for Water Utilities. Research Report No. 74. Water Quality Research Australia. Nova Scotia Environment (NSE). 2014. Environmental Quality Standards for Contaminated Sites, Rationale and Guidance Document. Version 1.0, April 2014. 57 p. Nova Scotia Salmon Association (NSSA) Adopt-A-Stream Program. 2020. SandWand. Accessed Feb 10, 2023 [http://adoptastream.ca/project-design/sandwand] Nova Scotia Salmon Association (NSSA) NSLC Adopt-A-Stream Program. 2014. Walking the River: A Citizen’s Guide to Interpreting Water Quality Data. 43 p. Nova Scotia Salmon Association (NSSA) NSLC Adopt-a-Stream Program. 2015. Acid Rain. [http://www.nssalmon.ca/issues/acid-rain]. Ontario Ministry of the Environment (MOE). 1979. Rationale for the establishment of Ontario’s Provincial Water Quality Objectives. Queen’s Printer for Ontario. 236 p. Ontario Ministry of the Environment (MOE). 2008. Guidelines for Identifying, Assessing and Managing Contaminated Sediments in Ontario. Queen’s Printer for Ontario. 112 p. Sereda, J. M., Hudson, J. J., Taylor, W. D., and Demers, E. 2008. Fish as sources and sinks of nutrients in lakes. Freshwater Biology, 53(2), 278-289. Smith, S. A., and Bella, D. A. 1973. Dissolved oxygen and temperature in a stratified lake. Journal (Water Pollution Control Federation), 119-133. United States Environmental Protection Agency (US EPA). 2002. Volunteer Lake Monitoring: A Methods Manual. United States Environmental Protection Agency. 65 p. United States Geological Survey (USGS). 2014. Hypoxia in the Gulf of Mexico. [toxics.usgs.gov/hypoxia/] 30 Wetzel, R. G. 1990. Land-water interfaces: metabolic and limnological regulators. Internationale Vereinigung für theoretische und angewandte Limnologie: Verhandlungen, 24(1), 6-24. Vanni, M. J. 2002. Nutrient cycling by animals in freshwater ecosystems. Annual Review of Ecology and Systematics, 33(1), 341-370.